Dna extraction protocol from bacteria
AbstractGuanidine thiocyanate breakage of microorganisms has been the standard initial step in genomic DNA (gDNA) extraction of microbial DNA for two decades, despite the requirement for pretreatments to extract DNA from microorganisms other than Gram-negative bacteria. We report a quick and low-cost gDNA extraction protocol called EtNa that is efficient for bacteria and yeast over a broad range of concentrations. EtNa is based on a hot alkaline ethanol lysis.
The solution can be immediately centrifuged to yield a crude gDNA extract suitable for PCR, or it can be directly applied to a silica column for purification. Molecular biology techniques such as PCR, mass spectrometry, and sequencing have been optimized in order to speed up the detection and analysis of microorganisms ( 1). DNA extraction methods, although central to these procedures, have seen little progress since the introducti.
It dna extraction protocol from bacteria deals with common plasmid DNA procedures, including how to make and transform competent cells, how to culture and handle plasmid-containing cells, and commonly used techniques for analysis of genomic DNA. It has been sent to the author(s) of this protocol.